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Proteintech rabbit polyclonal anti human tsg101
Rabbit Polyclonal Anti Human Tsg101, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+human+tsg101/TSG101+Antibody/pmc12254413-154-29-36
Average 96 stars, based on 945 article reviews

rabbit polyclonal anti human tsg101 - by Bioz Stars, 2026-10

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Article Title: CD20-bearing extracellular vesicles are associated with prognostic biomarkers of patients with AIDS-NHL.
Article Snippet: Samples were then loaded into each well of a precast 4–12% Bis-Tris Mini Protein Gel (1.0 mm) (3.5 to 260 kDa) (Invitrogen, Carlsbad, CA, USA), run at 130 V, and then transferred to a Millipore Immobilin-FL PVDF Membrane (0.45 μm) using the Mini-Protean Tetra System (Bio-Rad, Hercules, CA, USA) at 100 V for 1 h. Membranes were then immersed in 100% methanol for 30 s, rinsed once in double distilled water, and then rinsed in 1X Intercept TBS Blocking Buffer (1X TBS) (LI-COR Biosciences, Lincoln, NE, USA) for 5 min and blocked in 1X TBS for 1 h at room temperature with gentle shaking.

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Article Title: CD20-bearing extracellular vesicles are associated with prognostic biomarkers of patients with AIDS-NHL
Article Snippet: Samples were then loaded into each well of a pre-cast 4–12% Bis-Tris Mini Protein Gel (1.0 mm) (3.5 to 260 kDa) (Invitrogen, Carlsbad, CA, USA), run at 130 V, and then transferred to a Millipore Immobilin -FL PVDF Membrane (0.45 μm) using the Mini-Protean Tetra System (Bio-Rad, Hercules, CA, USA) at 100 V for 1 h. Membranes were then immersed in 100% methanol for 30 s, rinsed once in double distilled water, and then rinsed in 1X Intercept TBS Blocking Buffer (1X TBS) (LI-COR Biosciences, Lincoln, NE, USA) for 5 min and blocked in 1X TBS for 1 h at room temperature with gentle shaking. .. Membranes were then incubated with the following primary antibodies: mouse monoclonal anti-human CD9 (Catalog No. 602.29 cl.11; Developmental Studies Hybridoma Bank, Iowa City, IA, USA) at 1:50 multiplexed with rabbit polyclonal anti-human TSG101 (Catalog No. 28283-1-AP; Proteintech, Rosemont, IL, USA) at 1:2,000; mouse monoclonal anti-human CD81 (clone 1D6) (Catalog No. NB10065805; Novus Biologicals, Centennial, CO, USA) at 1:100 multiplexed with rabbit polyclonal anti-human HSP70/HSPA1A (Catalog No. AF1663; R&D Systems, Minneapolis, MN, USA) at 1:1000; and anti-human CD63 (Catalog No. H5C6; Developmental Studies Hybridoma Bank, Iowa City, IA, USA). ..

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Journal: Biochemical and biophysical research communications

Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

doi: 10.1016/j.bbrc.2024.151269

Figure Lengend Snippet: Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.

Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).

Techniques: Western Blot, Marker, Isolation, Negative Control, Protein Concentration, BIA-KA, Electron Microscopy, Knockdown

Fig. 4. CHKA knockdown Inhibited Proliferation, Migration and invasion of glioma Cells by Blocking Exosome Secretion. A Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in DMSO and GW4869 group. (BC) The proliferation of glioma cells U87MG and U251 in DMSO and GW4869 group, the migration ability of glioma cells U87MG (DE) and U251 (DF) in DMSO and GW4869 group (Scale bars = 200 μm). GH The invasion ability of glioma cells U87MG and U251 in DMSO and GW4869 group(Scale bars = 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: Biochemical and biophysical research communications

Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

doi: 10.1016/j.bbrc.2024.151269

Figure Lengend Snippet: Fig. 4. CHKA knockdown Inhibited Proliferation, Migration and invasion of glioma Cells by Blocking Exosome Secretion. A Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in DMSO and GW4869 group. (BC) The proliferation of glioma cells U87MG and U251 in DMSO and GW4869 group, the migration ability of glioma cells U87MG (DE) and U251 (DF) in DMSO and GW4869 group (Scale bars = 200 μm). GH The invasion ability of glioma cells U87MG and U251 in DMSO and GW4869 group(Scale bars = 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001.

Techniques: Knockdown, Migration, Blocking Assay, Western Blot, Marker, Isolation

Fig. 6. CHKA attenuates exosomes release through Autophagy-Mediated mechanism AB Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U87MG cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. AC Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U251 cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. D Exosome protein concentration in the s shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 groups of glioma cells using the BCA assay. **P < 0.01, ***P < 0.001. Baf-A1: Bafilomycin A1.

Journal: Biochemical and biophysical research communications

Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

doi: 10.1016/j.bbrc.2024.151269

Figure Lengend Snippet: Fig. 6. CHKA attenuates exosomes release through Autophagy-Mediated mechanism AB Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U87MG cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. AC Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U251 cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. D Exosome protein concentration in the s shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 groups of glioma cells using the BCA assay. **P < 0.01, ***P < 0.001. Baf-A1: Bafilomycin A1.

Techniques: Western Blot, Marker, Isolation, Protein Concentration, BIA-KA

Separate assessment of CMA and mA activities in response to Na 2 S in AD293 cells. ( A ) Punctate accumulation of GAPDH-HT in AD293 cells transfected with control, LAMP2A, or TSG101 siRNA, followed by the treatment with Na 2 S (10 μM) for 24 h. Scale bars are 10 μm. siRNA-mediated knockdown of LAMP2A and TSG101 was confirmed by immunoblotting . ( B ) Quantitative analyses of GAPDH-HT puncta shown in A. Numbers in the columns represent the numbers of observed cells. ** p < 0.01, *** p < 0.001 (Dunn’s multiple comparisons test).

Journal: Cells

Article Title: D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation

doi: 10.3390/cells11071230

Figure Lengend Snippet: Separate assessment of CMA and mA activities in response to Na 2 S in AD293 cells. ( A ) Punctate accumulation of GAPDH-HT in AD293 cells transfected with control, LAMP2A, or TSG101 siRNA, followed by the treatment with Na 2 S (10 μM) for 24 h. Scale bars are 10 μm. siRNA-mediated knockdown of LAMP2A and TSG101 was confirmed by immunoblotting . ( B ) Quantitative analyses of GAPDH-HT puncta shown in A. Numbers in the columns represent the numbers of observed cells. ** p < 0.01, *** p < 0.001 (Dunn’s multiple comparisons test).

Article Snippet: Anti-human LAMP2A rabbit polyclonal antibody (#ab125068) was obtained from AbCam (Cambridge, United Kingdom).

Techniques: Transfection, Control, Knockdown, Western Blot

Effect of Na 2 S on the amounts of Nrf2 and CMA-related proteins in AD293 cells. ( A ) Immunoblot analyses of Nrf2 and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 4 and 24 h. ( B ) Quantitative analyses of Nrf2 amounts from the immunoblot results shown in A. ( C ) Immunoblot analyses of CMA-related proteins (LAMP2A, Hsc70) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( D ) Quantitative analyses of LAMP2A and Hsc70 amounts from the immunoblot results shown in C. ( E ) Immunoblot analyses of a CMA/mA substrate (MEF2D) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( F ) Quantitative analysis of MEF2D amount from the immunoblot results shown in F. Whole blot images are presented in . Amounts of β-actin were used as internal controls for quantification. Numbers in the columns represent the number of samples. * p < 0.05, *** p < 0.001 (unpaired t -test).

Journal: Cells

Article Title: D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation

doi: 10.3390/cells11071230

Figure Lengend Snippet: Effect of Na 2 S on the amounts of Nrf2 and CMA-related proteins in AD293 cells. ( A ) Immunoblot analyses of Nrf2 and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 4 and 24 h. ( B ) Quantitative analyses of Nrf2 amounts from the immunoblot results shown in A. ( C ) Immunoblot analyses of CMA-related proteins (LAMP2A, Hsc70) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( D ) Quantitative analyses of LAMP2A and Hsc70 amounts from the immunoblot results shown in C. ( E ) Immunoblot analyses of a CMA/mA substrate (MEF2D) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( F ) Quantitative analysis of MEF2D amount from the immunoblot results shown in F. Whole blot images are presented in . Amounts of β-actin were used as internal controls for quantification. Numbers in the columns represent the number of samples. * p < 0.05, *** p < 0.001 (unpaired t -test).

Article Snippet: Anti-human LAMP2A rabbit polyclonal antibody (#ab125068) was obtained from AbCam (Cambridge, United Kingdom).

Techniques: Western Blot

Effect of long-term treatment with D-cysteine on the amounts of Nrf2- and CMA-related proteins in cerebellar lysates from ICR mice. ( A ) Immunoblot analyses of Nrf2, NQO1, LAMP2A, Hsc70, MEF2D, and β-actin in cerebellar lysates from ICR mice daily treated with saline (Sal) and D-cysteine (100 mg/kg/day) for 10 weeks. Whole blot images are presented in . ( B ) Quantitative analyses of the amounts of Nrf2, NQO1, LAMP2A, Hsc70, and MEF2D shown in A. Amounts of β-actin were used as internal controls for the quantification. * p < 0.05, ** p < 0.01 vs. saline-treated mice (unpaired t -test, n = 5 in both saline- and D-cysteine-treated mice).

Journal: Cells

Article Title: D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation

doi: 10.3390/cells11071230

Figure Lengend Snippet: Effect of long-term treatment with D-cysteine on the amounts of Nrf2- and CMA-related proteins in cerebellar lysates from ICR mice. ( A ) Immunoblot analyses of Nrf2, NQO1, LAMP2A, Hsc70, MEF2D, and β-actin in cerebellar lysates from ICR mice daily treated with saline (Sal) and D-cysteine (100 mg/kg/day) for 10 weeks. Whole blot images are presented in . ( B ) Quantitative analyses of the amounts of Nrf2, NQO1, LAMP2A, Hsc70, and MEF2D shown in A. Amounts of β-actin were used as internal controls for the quantification. * p < 0.05, ** p < 0.01 vs. saline-treated mice (unpaired t -test, n = 5 in both saline- and D-cysteine-treated mice).

Article Snippet: Anti-human LAMP2A rabbit polyclonal antibody (#ab125068) was obtained from AbCam (Cambridge, United Kingdom).

Techniques: Western Blot, Saline

Primary antibodies used for Western blotting.

Journal: Scientific Reports

Article Title: Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species

doi: 10.1038/s41598-020-74444-8

Figure Lengend Snippet: Primary antibodies used for Western blotting.

Article Snippet: Human TSG101 Antibody (CSB-PA060017) , Polyclonal Rabbit IgG , Cusabio Technology LLC , 1:1000.

Techniques: Western Blot

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